Sampletype i-sep® SQ
Quantitative release and separation of DNA in one system
Sampletype i-sep® SQ are developed for quantitative DNA extraction procedures to provide DNA lysates of highest quality. The proprietary filter material allows the sample lysis and separation of DNA in the same device without any manual transfer of the substrate or additional pipetting steps. The novel column system is ideally suitable for DNA extraction procedures involving a diversity of specimens such as clinical or forensic sample material.
- Improved yield, reproducibility, and overall purity of isolated DNA
- Improved process flow
- No manual transfer of the substrate
- No extra pipetting steps
- Lysis and separation in the same device without extra pipetting steps
- Quantitative recovery of lysate
- Closed system that minimizes the risk of cross-contaminations and mix-ups
- Compatible to any consecutive DNA isolation, both manual and automated
Product description
The quantity and quality of genomic DNA extracted from any sample can greatly impact the success of the down-stream analysis and the overall quality of the final result. This is particular the case for patient samples or field specimens which might be limited in quantity, may be environmentally exposed, and may require purification from difficult substrates containing PCR inhibitors. The overall efficiency is improved, and the yield of isolated DNA is optimised by carrying out the lysis directly in the column with subsequent quantitative recovery of the lysis buffer.
Sampletype i-sep® SQ are based on the established mini spin-column format containing a special filter compartment which prevents flow-through of different kinds of liquid without any centrifugal force. They retain solutions within the column even during heat incubation and shaking. Upon centrifugation, the solution passes the column into the collection tube.
Sampletype i-sep® SQ are recommended in particular for clinical and forensic specimens.
The commonly used spin basket method , in comparison to the novel Sampletype method, requires the transfer of substrate using sterile tweezers. Furthermore the tube must be opened several times increasing the risk of cross-contamination.
The new Sampletype i-sep® SQ allows the lysis and effective substrate removal in an all-in-one assembly.No transfer of the substrate is required , minimising hands on time and reducing the risk of cross-contamination and sample transposition events.
Now available +++ Now available +++ Now available +++
| Ordering information | Cat. No. | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 500 spin column filters + 500 collection tubes | 61-00201-0500 |
Product information
AppNote 102
