Mentype® Chimera®

Mentype® Chimera® PCR Amplification Kit was developed as a multiplex application for chimerism monitoring after bone marrow transplantation.
In one PCR reaction, the twelve polymorphic autosomal Short Tandem Repeat (STR) loci D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D21S2055, and SE33 (ACTBP2) as well as the gender-specific marker Amelogenin are amplified simultaneously. The primers are fluorescence-labeled with 6-FAMTM, BTG or BTY.

The STR loci were chosen with respect to their high discrimination power. Most of the highly polymorphic markers were validated by monitoring chimerism of over 200 HLA-matched related donor-recipient-pairs (Thiede et al., 2004).

Mentype® Chimera® - the reliable semi-quantitative solution for chimerism analysis.

The Mentype® Chimera® PCR Amplification Kit is very sensitive. The detection limit is less than 200 pg genomic DNA. Recipient fractions of less then 5 % are still detectable, so that residual cells or recurrence can be monitored.

 

Ordering information                 Cat. No.
25 Reactions                 45-13210-0025
100 Reactions                 45-13210-0100
400 Reactions                 45-13210-0400

Template Files
undefined Genotyper®
undefined GeneMapper ID
undefined GeneMapper™ ID-X
Information to the template files find here.

Your contact
Dr Sophia Mersmann
Tel.: +49 351 8838 432
Fax: +49 351 8838 403
info@biotype.de