Humantype Chimera®
Humantype Chimera® PCR Amplification Kit was developed as multiplex application for chimerism monitoring after bone marrow transplantation.
In one PCR reaction, the twelve polymorphic autosomal Short Tandem Repeat (STR) loci D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D21S2055, and SE33 (ACTBP2) as well as the gender-specific marker Amelogenin are amplified simultaneously. The primers are fluorescence labled with 6-FAM, HEX, NED.
The STR loci were chosen with respect of their high discrimination power. Most of the highly polymorphic markers were validated by monitoring chimerism over 200 HLA-matched related donor-recipient-pairs (Thiede et al., 2004).
Humantype Chimera® - currently the most sensitive solution for chimerism analysis.
The Humantype Chimera® PCR Amplification Kit is very sensitive. The detection limit is less than 200 pg genomic DNA. Donor fractions of less then 5% are still detectable, so that residual cells or recurrence are early detectable.
| Ordering information | Cat. No. | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 25 reactions | 31-13110-0025 | ||||||||
| 100 reactions | 31-13110-0100 | ||||||||
| 400 reactions | 31-13110-0400 |
