QPCR POLE Mutation Analysis Kit
Efficient Screening of Relevant POLE Mutations for Clinical Research
The POLE gene encodes the catalytic subunit of DNA polymerase epsilon, which is responsible for proofreading during DNA replication. Mutations in the POLE exonuclease domain are examined for the staging of endometrial tissue samples in clinical research.
The QPCR POLE Mutation Analysis Kit detects six major mutations and can distinguish five according to international guidelines (S31, ESMO2). This kit is designed for common qPCR instruments and integrates seamlessly into your existing workflow, making it a valuable addition to your research arsenal.
1 S3-Leitlinie Endometriumkarzinom, AWMF-Registernummer: 032/034-OL
2 https://doi.org/10.1016/j.annonc.2022.05.009
Mutation Coverage
| Exon | Amino acid change |
|---|---|
| 9 | P286R S297F |
| 13 | V411L (G > C, G > T) |
| 14 | A456P S459F |
Product Features
- High sensitivity - 1 % VAF (2 % tumor content)
- Low DNA input - 8 ng/sample (2 x 4 ng)
- Fast results - 2 h from DNA to result
- No bioinformatics - Intuitive data analysis using instrument software, no further calculation required
- No specific equipment - Use your qPCR instrument (6-FAM, VIC, ROX, Cy5 detection required)
Optimize Your Workflow for POLE Testing
The QPCR POLE Mutation Analysis Kit takes advantage of qPCR testing for a fast and targeted screening. The test kit is designed to be compatible with widely used qPCR instruments and integrates seamlessly into existing workflows to streamline processes and optimize resources.
Product Specification
| Cat No. | 35-14900-0100 |
|---|---|
| Regulatory status | RUO* |
| Tissue | Endometrial tissue |
| Sample type | DNA from FFPE tissue |
| Mutation variants | 6 |
| Kit size | 100 reactions / 2 x 50 tests |
| Platform | Real-Time PCR |
*RUO - Research Use Only products must be validated by the customer with clinically relevant material for diagnostic purposes.
