Mentype® DIPscreen

Tailored chimerism monitoring


  • High discrimination power
  • Low DNA input for high sensitivity ≥ 2 %
  • Allows high throughput genotyping and monitoring

Mentype® DIPscreen is the optimal tool for a cost-effective start into sensitive chimerism analysis. The kit is used for initial genotyping of recipient and donor samples and can also be used for the routine chimerism monitoring with a sensitivity of up to 2 %. A subsequent highly sensitive chimerism quantification can be performed either with the qPCR assays Mentype® DIPquant or the broad applicable digital PCR kit Mentype® DigitalQuant (RUO). These combined approaches provide flexible and reliable chimerism monitoring adaptable to your needs.



DIP Locus: AM X, AM Y, HLD106, HLD70, HLD84, HLD103, HLD104, HLD116, HLD112, HLD307, HLD310, HLD110, HLD133, HLD79, HLD105,HLD140, HLD163

Chromosomal localization: Xp22.1-22.3, Yp11.2, 16q13, 6q16.1, 8q24.12, 12q23.1, 13q32.1, 18p11.22, 17p12, Xp11.23, 2p22.3, 16q22.1, 3p22.1, 7q31.2, 14q24.3, 3q23, 12q24.31

BTG Panel

DIP Locus: HLD91, HLD23, HLD88, HLD101, HLD67, HLD301, HLD53, HLD97, HLD152, HLD128, HLD134, HLD305

Chromosomal localization:11q14.1, 18p11.32, 9q22.33, 15q26.1, 5q33.3, 17q21.32, 3q22.1, 13q13.1, 16p13.2, 1q31.3, 5q11.2, 20q11.22

BTY Panel

DIP Locus: HLD48, HLD114, HLD304, HLD131, HLD38, HLD82

Chromosomal localization: 2q11.2, 17p13.2, 9q34.3, 7q36.2, 1q32.2, 7q21.3

Product Specifications

  • Panel
  • 33 DIP Loci + Amelogenin
  • Reactions
  • 1 Multiplex PCR reaction per sample
  • PCR controls
  • 2 included (PC, NTC)
  • Sample input
  • 1-2 ng gDNA from peripheral blood
  • Sensitivity
  • ≥ 2 %
  • Turnaround time
  • ~ 4 h after nucleic acid preparation
  • Detection
  • Qualitative genotyping, Semiquantitative chimerism detection
  • To be used with
  • Standard thermal cycler + Thermo Fisher Genetic Analyzer
  • Data analysis
  • ChimerisMonitor (RUO), GeneMapperTM ID/ ID-X + specific templates

Scientific Background

Analysis of molecular chimerism resulting from allogeneic stem cell transplantation has become a well-established method to control the course of transplant engraftment and to assess the risk of threatening relapse. Molecular chimerism analysis can be performed on diverse DNA-sequence motifs, of which biallelic short insertion/deletion polymorphisms (DIPs, INDELs) offer substantial benefits. Polymerase-mediated amplification of DIP-markers does not result in formation of stutter peaks that can hamper clear analysis. Moreover, these polymorphisms are best suited for allele-specific quantitative approaches. Mentype® DIPscreen is a DIP-based chimerism analysis and therefore accounts for an unambiguous donor/recipient differentiation and highly clear chimerism monitoring.

Product References

  1. Ferreras, C. et al. Results of phase 2 randomized multi-center study to evaluate the safety and efficacy of infusion of memory T cells as adoptive therapy in severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia and/or lymphopenia (RELEASE NCT04578210). Cytotherapy 26, 25-35, (2024)
  2. Al-Akioui Sanz, K. et al. Familial CD45RA– T cells to treat severe refractory infections in immunocompromised patients. Front. Med. 10. (2023)
  3. Huyveneers, L. E. P. et al. Autopsy Study Defines Composition and Dynamics of the HIV-1 Reservoir after Allogeneic Hematopoietic Stem Cell Transplantation with CCR5D32/D32 Donor Cells. Viruses 14. (2022)
  4. Pérez-Martínes, A. et al. Phase I dose-escalation single centre clinical trial to evaluate the safety of infusion of memory T cells as adoptive therapy in COVID-19 (RELEASE). EClinicalMedicine 39. (2021)
  5. Fortschegger, M. et al. Detection and Monitoring of Lineage-Specific Chimerism by Digital Droplet PCR-Based Testing of Deletion/Insertion Polymorphisms. Biol Blood Marrow Transplant 26, 1218-1224, (2020)
  6. Gómez-García, L. M. et al. A phase II clinical trial of infusing haploidentical K562-mb-IL15-41BBL activated and expanded Natural Killer cells as consolidation therapy for pediatric acute myeloblastic leukemia. ESS Open Archive. DOI: 10.22541/au.160192968.85891275/v1 (2020)
  7. Navarro-Bailón, A et al. Short Tandem Repeats (STRs) as Biomarkers for the Quantitative Follow-Up of Chimerism after Stem Cell Transplantation: Methodological Considerations and Clinical Application. Genes 11, 993. (2020)
  8. Cechova, H. et al. Suitable Molecular Genetic Methods for the Monitoring of Cell Chimerism. Rare Diseases. IntechOpen. (2019)
  9. Salgado, M. et al. Mechanisms That Contribute to a Profound Reduction of the HIV-1 Reservoir After Allogeneic Stem Cell Transplant. Annals of Internal Medicine 169, 674-683. (2018)
  10. Sellmann, L. et al. Diagnostic value of highly-sensitive chimerism analysis after allogeneic stem cell transplantation. Bone Marrow Transplant 53, 1457-1465. (2018)
  11. Navarro-Bailón, A. et al. A Novel Quantitative PCR Approach Targeting Insertion/Deletion Polymorphisms (Indel-PCR) for Chimerism Quantification: Finally High Sensitivity and Quantification Capacity Together. Blood 126, 4227. (2015)
  12. Willasch, A. M. et al. Monitoring of Hematopoietic Chimerism after Transplantation for Pediatric Myelodysplastic Syndrome: Real-Time or Conventional Short Tandem Repeat PCR in Peripheral Blood or Bone Marrow? Biol Blood Marrow Transplant 20, 1918-1925. (2014)

Ordering Information

Mentype® DIPscreen

Größe: 25 Reaktionen
Artikelnummer: 45-45410-0025
Status: CE-IVD

Mentype® DIPscreen

Größe: 100 Reaktionen
Artikelnummer: 45-45410-0100
Status: CE-IVD

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