Mentype® AMLplexQS CE-IVD
Mentype® AMLplexQS represents the ideal screening tool to detect gene aberrations associated with acute myeloid leukaemia (AML). One reaction of this multiplex-PCR application provides analysis of 11 gene-fusions, and in total 34 transcript variants. RNA isolated from blood or bone-marrow should be transcribed to cDNA and, subsequently, can be transferred to the kit's optimised components for multiplex analysis. Primers are fluorescence-labeled with 6-FAMTM, BTG, or BTY allowing a fast and sensitive analysis by capillary electrophoresis.
Gene fusion | Aberration | Variants |
|---|---|---|
AML1-ETO | t(8;21)(q22;q22) | - |
BCR-ABL | t(9;22)(q34;q11) | e1a2/3, b2a2/3, b3a2/3 |
CALM-AF10 | t(10;11)(p13;q14) | AF10_240-CALM_1987 |
AF10_240-CALM2092 | ||
CBFB-MYH11 | inv(16)(p13;q22) | Typ A, B, C, D, E, F, G, H, I, J |
DEK-CAN | t(6;9)(p23;q34) | - |
MLL-AF6 | t(6;11)(q27;q23) | - |
MLL-AF9 | t(9;11)(p22;q23) | 6A_(THP1), 7A_(10A), |
8A_(MM6), 6B_(9B) | ||
MLL-ELL | t(11;19)(q23;p13.1) | e10e2/3 |
MLL-PTD | part. tandem duplication | e9e3, e10e3, e11e3 |
NPM1-MLF1 | t(3;5)(q25.1;q34) | - |
PML-RARA | t(15;17)(q22;q21) | bcr1 (PR-L), bcr2 (PR-V), |
bcr3 (PR-S) |
The amount of isolated RNA and quality of the reverse transcription reaction is displayed by the enclosed ABL-control. Successful multiplex amplification is monitored by a control-amplification of the internal quality sensor “QS”. Furthermore, a cDNA carrying the AML1-ETO translocation is enclosed as a positive control.
Mentype® AMLplexQS represents your reliable diagnostics for precise, highly sensitive and routine-fit analysis of fusion gene transcripts in acute myeloid leukaemia.
| Ordering information | Cat. No. | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 25 reactions | 45-31220-0025 | ||||||||
| 100 reactions | 45-31220-0100 | ||||||||
| 400 reactions | 45-31220-0400 |
GeneMapper
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